The applied biosystems taqpath covid19 combo kit is a fast, highly sensitive multiplex diagnostic solution that contains both the assays and controls needed for the realtime pcr detection of rna from the sarscov2 virus. Applied biosystems 75007500 fast realtime pcr system getting started guide for genotyping experiments v preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems 75007500 fast realtime pcr system 75007500 fast system. Applied biosystems 7500 fast realtime pcr system thermo. The applied biosystems 730075007500 fast realtime pcr system site preparation guide provides the information you need to fully prepare your site for the arrival and installation of the applied biosystems 730075007500 fast real.
Applied biosystems thermal cycler fleet control software applied. Applied biosystems 7500 fast realtime pcr system, desktop. Each experiment file is a virtual representation of a. Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Field application specialist doug rains offers advices for fixing common software setup mistakes when performing realtime pcr. Faster ramp rates enable rapid results without compromising extension times or assay quality. Applied biosystems stepone and steponeplus realtime pcr. Specially designed fast block ensures thermal uniformity at top speeds. The applied biosystems analysis software can import and analyze experiment files eds and sds that are generated by various thermo fisher scientific realtime qpcr instruments.
Applied biosystems 7500 fast real time pcr system software. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. The applied biosystems 7500 fast dx realtime pcr instrument with sds software is a realtime nucleic acid amplification and fivecolor fluorescence detection system available for in vitro diagnostic use. Applied biosystems 7500 fast and 7500 realtime pcr. Realtime pcr data analysis thermo fisher scientific us. Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for 23 presenceabsence experiments notes. Applied biosystems stepone realtime pcr system, laptop. Detection of viral rna not only aids in the diagnosis of illness but also provides. Applied biosystems 7500 realtime pcr system biocompare. Fully optimized for fast cycling, the 7500 fast delivers highquality results in as little as 30 minutes. Prior to downloading any software to a computer used for instrument controloperation, please.
Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through. Prior to downloading any software to a computer used for instrument controloperation, please consult. The stepone realtime pcr system is a 48well, lowthroughput realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems 7500 realtime pcr system thermo fisher. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. Which version of the applied biosystems 7500 realtime pcr. Which version of the applied biosystems 7500 realtime pcr system software can be used to run the investigator. Used abi stepone qpcr on sale at the lab world group. Applied biosystems quantstudio 1 realtime pcr systems.
Flow cytometry experience better data, faster speed, and simplicity with the invitrogen attune acoustic focusing cytometer. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Applied biosystems steponeplus realtime pcr system. Applied biosystems pioneered the development of realtime pcr in 1995, enabling highthroughput, quantitative gene expression. Real time pcr model applied biosystems 7500 now on sale at the lab world group.
Taqpath covid19 multiplex diagnostic solution thermo. These factorycalibrated systems make it easy to step up to highperformance qpcr. The allelic discrimination plot created by the desktop software figure. Compatible realtime pcr system data the applied biosystems analysis software can. Applied biosystem 7500 fast real time pcr system andbio. The quantstudio 5 realtime pcr system is compact in form, factory calibrated and is delivered in userfriendly packaging to enable fast and simple installation for immediate use. The applied biosystems 7500 fast realtime pcr system offers maximum performance in the minimum time. The 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. The standard genotyping experiment method was used on the applied biosystems quantstudio 5 realtime pcr system. Applied biosystems dna extract all reagents kit and used directly in qpcr by adding applied biosystems taqman gtxpress master mix. Help from within applied biosystems 75007500 fast realtime pcr software v2. Applied biosystems 7500 software setup for the quantifast.
This remarkably simple realtime pcr system is designed with an lcd touchscreen and a userfriendly interface that brings the power of genetic studies to researchers new to realtime pcr fig 1. How to use stepone plus qpcr machine by applied biosystems. The intuitive software and protocol wizards help guide new users through their realtime pcr experiments. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. With either 3 or 6 independent temperature zones, the applied biosystems veriflex blocks within the quantstudio pro instruments provide flexibility to run multiple. Applied biosystems 7500 realtime pcr system, desktop. Applied biosystems 75007500 fast thermo fisher scientific. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well.
Designed for compatibility with applied biosystems taqman assays and the flexibility of unlimited realtime chemistry choices, we make it easy for you to get started with your experiments. The applied biosystems analysis software can import and analyze data generated by any of the supported instruments listed in the following table. Applied biosystems 7500 realtime pcr system thermo. Applied biosystems 7500 fast dx realtime pcr instrument. Applied biosystems realtime pcr instruments thermo. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. Paired up with the software the steponeplus is capable of collecting melting curve data for high resolution melt also known as hrm. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. The applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. For further information contact the director of licensing, applied biosystems, 850 lincoln. A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software. The applied biosystems 7500 and 7500 fast realtime pcr systems are covered by us patent no. Applied biosystems relative quantitation analysis module.
Set up, run, and analyze experiments using the stepone and steponeplus systems. The applied biosystems 7500 rt pcr machine operates using a 96 well plate, a reaction volume range of 20100ul with a dynamic range of 9 logs, a block ramp rate of. Applied biosystems 7500 getting started pdf download. The software versions listed in the table represent only those tested for use with the applied biosystems software. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast pcfree or networked operation.
With our applied biosystems realtime pcr platforms, you get true value with excellent performance, reliability, and worldclass support. Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems. Disclaimer the text above is not a recommendation to uninstall realtime pcr software v2. Applied biosystems quantstudio 5 realtime pcr system, 96. The overall size and footprint of the abi 7500 was made more compact. Every consumable run on a thermo fisher scientific realtime qpcr instrument requires the creation of one or more experiment files that store the associated data. Notes applied biosystems 75007500 fast realtime pcr system getting started guide for standard curve experiments. To verify your client connection, you need a valid user id.
These 96 well realtime pcr systems offers four color optical recording allowing for faster measurement. Robust and reliable applied biosystems thermal cyclers and pcr enzymes and reagents help you achieve success for virtually any application. Applied biosystems stepone steponeplus realtime pcr. The applied biosystems steponeplus is a realtime pcr system designed to meet your needs. The steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems quantstudio 6 and 7 pro realtime pcr systems are able to detect differences in target quantity as small as 1. Applied biosystems 7500 and 7500 fast software version 2.
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